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zeiss axioobserver 7  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss axioobserver 7
    Zeiss Axioobserver 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zeiss+axioobserver+7/Microscope+stand+Axio+Observer+7/pm42867703-100-5-5
    Average 98 stars, based on 1043 article reviews
    zeiss axioobserver 7 - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Imaging:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics.
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide- field fluorescence microscope with light- emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide-field fluorescence microscope with light-emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Fluorescence:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics.
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide- field fluorescence microscope with light- emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide-field fluorescence microscope with light-emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Microscopy:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics.
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide- field fluorescence microscope with light- emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Article Title: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
    Article Snippet: .. Samples were imaged at X63 magnification on a Zeiss AxioObserver 7 inverted fluorescent microscope with the Zen Blue 2.3 software. ..

    Article Title: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
    Article Snippet: .. Samples were imaged on a Zeiss AxioObserver 7 inverted fluorescent microscope with the Zen Blue 2.3 software and 20-30 images were taken per coverslip/well at X63 magnification, using DAPI and tubulin staining as a guidance for the presence of cells. .. PLA particles were quantified within tubulin-labelled cells, then normalised to the number of cells using Fiji (Fiji Is Just ImageJ, version 2.3.0/1.53t, National Institutes of Health ).

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. The samples were mounted in Prolong Gold with DAPI (Invitrogen) on glass slides and left to dry in the dark for 24 hours before imaging on a Zeiss AxioObserver 7 inverted wide-field fluorescence microscope with light-emitting diode (LED) illumination (SpectrX, Lumencor) and sCMOS ORCA Flash 4.0 V3 717 (Hamamatsu). ..

    Article Title: A Novel Single-Color FRET Sensor for Rho-Kinase Reveals Calcium-Dependent Activation of RhoA and ROCK
    Article Snippet: Briefly, two Zeiss AxioObserver Microscopes (Carl Zeiss MicroImaging, Oberkochen, Germany) equipped for widefield imaging were used to gather fluorescence anisotropy images. .. The Zeiss AxioObserver Z1 microscope was controlled using Zeiss AxioVision 4.7.2 software, and the Zeiss AxioObserver 7 was controlled using Zeiss Zen Blue 2.6 software. .. Images were collected with a 20X 0.75 NA objective lens on the Zeiss AxioObserver Z1 microscope.

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Article Title: Lamellipodia dynamics and microrheology in endothelial cell paracellular gap closure
    Article Snippet: Phalloidin was purchased from Invitrogen (Carlsbad, CA). .. Mechanical measurements were performed using a JPK NanoWizard 4 AFM (Bruker) run by a JPK Vortis advanced control station (also from Bruker), integrated with the Zeiss AxioObserver 7 inverted optical microscope described above, and equipped with the heating stage, sample stage, and gas mixer already described. .. Mechanical measurements were performed using a JPK NanoWizard 4 AFM (Bruker) run by a JPK Vortis advanced control station (also from Bruker), integrated with the Zeiss AxioObserver 7 inverted optical microscope described above, and equipped with the heating stage, sample stage, and gas mixer already described.

    Software:

    Article Title: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
    Article Snippet: .. Samples were imaged at X63 magnification on a Zeiss AxioObserver 7 inverted fluorescent microscope with the Zen Blue 2.3 software. ..

    Article Title: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
    Article Snippet: .. Samples were imaged on a Zeiss AxioObserver 7 inverted fluorescent microscope with the Zen Blue 2.3 software and 20-30 images were taken per coverslip/well at X63 magnification, using DAPI and tubulin staining as a guidance for the presence of cells. .. PLA particles were quantified within tubulin-labelled cells, then normalised to the number of cells using Fiji (Fiji Is Just ImageJ, version 2.3.0/1.53t, National Institutes of Health ).

    Article Title: A Novel Single-Color FRET Sensor for Rho-Kinase Reveals Calcium-Dependent Activation of RhoA and ROCK
    Article Snippet: Briefly, two Zeiss AxioObserver Microscopes (Carl Zeiss MicroImaging, Oberkochen, Germany) equipped for widefield imaging were used to gather fluorescence anisotropy images. .. The Zeiss AxioObserver Z1 microscope was controlled using Zeiss AxioVision 4.7.2 software, and the Zeiss AxioObserver 7 was controlled using Zeiss Zen Blue 2.6 software. .. Images were collected with a 20X 0.75 NA objective lens on the Zeiss AxioObserver Z1 microscope.

    Staining:

    Article Title: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
    Article Snippet: .. Samples were imaged on a Zeiss AxioObserver 7 inverted fluorescent microscope with the Zen Blue 2.3 software and 20-30 images were taken per coverslip/well at X63 magnification, using DAPI and tubulin staining as a guidance for the presence of cells. .. PLA particles were quantified within tubulin-labelled cells, then normalised to the number of cells using Fiji (Fiji Is Just ImageJ, version 2.3.0/1.53t, National Institutes of Health ).

    Immunofluorescence:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Confocal:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Control:

    Article Title: Lamellipodia dynamics and microrheology in endothelial cell paracellular gap closure
    Article Snippet: Phalloidin was purchased from Invitrogen (Carlsbad, CA). .. Mechanical measurements were performed using a JPK NanoWizard 4 AFM (Bruker) run by a JPK Vortis advanced control station (also from Bruker), integrated with the Zeiss AxioObserver 7 inverted optical microscope described above, and equipped with the heating stage, sample stage, and gas mixer already described. .. Mechanical measurements were performed using a JPK NanoWizard 4 AFM (Bruker) run by a JPK Vortis advanced control station (also from Bruker), integrated with the Zeiss AxioObserver 7 inverted optical microscope described above, and equipped with the heating stage, sample stage, and gas mixer already described.



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